ARPHA Preprints, doi: 10.3897/arphapreprints.e102387
Molecular characterisation of three Ixodes (Pholeoixodes) species (Ixodida, Ixodidae) and the first record of Ixodes (Pholeoixodes) kaiseri from Slovakia
expand article infoZuzana Krumpálová‡, Barbara Mangová§, Slávka Purgatová‡, Yuliya M. Didyk§, Mária Kazimírová§
‡ Constantine the Philosopher University, Faculty of Natural Sciences and Informatics, Nitra, Slovakia§ Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia

Corresponding author: Mária Kazimírová ( maria.kazimirova@savba.sk )

© Zuzana Krumpálová, Barbara Mangová, Slávka Purgatová, Yuliya M. Didyk, Mária Kazimírová.
This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Krumpálová Z, Mangová B, Purgatová S, Didyk YM, Kazimírová M (2023) Molecular characterisation of three Ixodes (Pholeoixodes) species (Ixodida, Ixodidae) and the first record of Ixodes (Pholeoixodes) kaiseri from Slovakia. ARPHA Preprints. https://doi.org/10.3897/arphapreprints.e102387
Open Access
Abstract
A study of ticks on wildlife was carried out in the area of Levice, Bratislava, Stupava and Vrbovce (south-western Slovakia) during the years 2021-2022. Overall, 512 ticks were collected from 51 wild mammalian individuals of six species. In total, eight tick species were identified, namely Dermacentor reticulatus, D. marginatus, Haemaphysalis inermis, H. concinna, Ixodes ricinus, I. hexagonus and two Ixodes spp. Ixodes hexagonus were collected from Northern white-breasted hedgehogs (Erinaceus roumanicus), females belonging to Ixodes spp. were collected from a red fox (Vulpes vulpes) and nymphs from a Eurasian badger (Meles meles). Ixodes hexagonus and the Ixodes spp. were identified morphologically and molecularly based on sequences of fragments of two mitochondrial genes, COI and 16S rRNA. Molecular analysis of Ixodes spp. confirmed the identity of Ixodes kaiseri Arthur, 1957 and I. canisuga (Johnston, 1849). Sequence analyses show that the I. kaiseri isolate from Slovakia is identical to I. kaiseri isolates from Romania, Poland, Germany, Turkey, and Croatia. We demonstrate for the first time the presence of I. kaiseri in Slovakia using both morphological and molecular methods.
Keywords
European badger, Hedgehog, Ixodes canisuga, Ixodes kaiseri, Red fox, Ticks