The Galápagos ant fauna has long been understudied, with the last taxonomic summary being published almost a century ago. Here, we provide a comprehensive and updated overview of the known ant species of the Galápagos Islands with updated species distributions. The list is based on an extensive review of literature, the identification of more than 382,023 specimens deposited in different entomological collections, as well as recent expeditions to the islands. The fauna is composed of five subfamilies (Dolichoderinae, Dorilynae, Formicinae, Myrmicinae and Ponerinae), 22 genera and 53 species, though 3 of these species are considered dubious records. Finally, we provide an illustrated identification key of the species in the archipelago.