ARPHA Preprints, doi: 10.3897/arphapreprints.e108949

Karyotype and genome size variation in Delphinium subg. Anthriscifolium (Ranunculaceae)

Xiaoyu Luo^‡§, Tang-Jie Nie^|, Heng Liu^‡, Xue-Fei Ding^‡, Ying Huang^‡, Chunce Guo^‡, WenGen Zhang^‡

‡ Jiangxi Agricultural University, Nanchang, China§ Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Nanchang, China| Nanjing Forestry University, Nanjing, China

Corresponding author: Xiaoyu Luo ( xiaoyuluo202102@163.com )

Corresponding author: WenGen Zhang ( wgzhang@jxau.edu.cn )

This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation:

Abstract

Five taxa of Delphinium subg. Anthriscifolium have been karyologically studied through chromosome counting, chromosomal measurement, and karyotype symmetry. All studied specimens had a basic chromosome number of x = 8, D. anthriscifolium var. savatieri, D. anthriscifolium var. majus, D. ecalcaratum, and D. callichromum were diploid with 2n = 16, while D. anthriscifolium var. anthriscifolium was tetraploid with 2n = 32. Monoploid chromosome sets of the investigated diploid taxa contained 1 metacentric chromosome, 3 submetacentric chromosomes, and 4 subtelocentric chromosomes. Higher interchromosomal asymmetry (CV_CL) was present in D. ecalcaratum and D. callichromum than in other taxa. The highest levels of intrachromosomal asymmetry (M_CA) and heterogeneity in centromere position (CV_CI) were found in D. anthriscifolium var. majus. Diploid and tetraploid genome sizes varied by 3.02–3.92 pg and 6.04–6.60 pg, respectively. Karyotype and genome size of D. anthriscifolium var. savatieri, D. anthriscifolium var. majus, D. callichromum, and D. ecalcaratum were reported for the first time.

Keywords

Karyotype, genome size, Delphinium, Ranunculaceae, ploidy