ARPHA Preprints, doi: 10.3897/arphapreprints.e117037
New data on trophic associations of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae) and lemurs (Primates, Lemuroidea) in Madagascar
expand article infoAndrey V. Frolov, Maria S. Vishnevskaya§|, Heidi Viljanen, Olivier Montreuil#, Lilia A. Akhmetova|
‡ Zoological Institute RAS, St. Petersburg, Russia§ Saint Petersburg State University, Saint Petersburg, Russia| Zoological Institute RAS, Saint Petersburg, Russia¶ Finnish Museum of Natural History, Helsinki, Finland# Département AVIV, UMR 7179, MECADEV, MNHN/CNRS, Entomologie, Paris, France
Open Access
Abstract

Resource use and diet specialization of Madagascan dung beetles has been little studied especially concerning the possible associations between specific dung beetle and lemur species. Pilot studies have demonstrated that amplicon metagenomics is a promising tool for the lemur inventories. In the present contribution, we report new results of the gut content analysis of three endemic Madagascan dung beetles species: Helictopleurus clouei (Harold), Epilissus apotolamproides (Lebis) and Nanos dubitatus (Lebis). Amplicon metagenomics revealed trophic associations of these species with Eulemur sanfordi (Archbold), Eu. fulvus (É.Geoffroy Saint-Hilaire), and Cheirogaleus crossleyi (Grandidier), respectively. The reads of other mammal species, revealed by the analysis, including putative contaminations, are discussed.

Keywords
Scarabaeines, coprophagy, gut content analysis, food chains, next-generation sequencing, amplicon metagenomics