ARPHA Preprints, doi: 10.3897/arphapreprints.e125475

The ASV registry: a place for ASVs to be

Christian Bräunig^‡, Sandra Meid^§, Björn Quast^§, Vera Rduch^§, Peter Grobe^§

‡ Leibniz-Institute for the Analysis of Biodiversity Change, Bonn, Germany§ Leibniz Institute for the Analysis of Biodiversity Change - Museum Koenig Bonn, Bonn, Germany

Corresponding author: Christian Bräunig ( c.braeunig@leibniz-lib.de )

This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Bräunig C, Meid S, Quast B, Rduch V, Grobe P (2024) The ASV registry: a place for ASVs to be. ARPHA Preprints. https://doi.org/10.3897/arphapreprints.e125475

Abstract

Despite the effectiveness of DNA metabarcoding for gaining insights into biodiversity and environmental species composition, a centralized management and storage option including easy accessibility of already published data is lacking. Since most data is published as supplementary material or in private repositories, DNA metabarcoding has a huge untapped potential to be used for analysis across multiple taxa, sample locations or multiple research projects. We developed a platform to register, manage and identify amplicon sequence variants (ASVs) or zero-radius OTUs (ZOTUs), respectively, against several barcode reference datasets. Moreover, ASV tables can be uploaded, managed, versioned, and published with DOIs thus contributing to the full research Data Life Cycle.

Keywords

amplicon sequence variant, barcode reference databases, biodiversity monitoring, data storage, DNA metabarcoding, operational taxonomic unit, taxon annotation