ARPHA Preprints, doi: 10.3897/arphapreprints.e128742
Steps of DNA extraction for molecular analysis of microalgae communities - a simple guide for beginners
Tena Radočaj‡, Oliver Barić‡, Serena Rasconi§, Irena Vardić|, Jurica Jug-Dujaković¶, Ana Gavrilović#‡ University of Zagreb, Faculty of Agriculture, Zagreb, Croatia§ Université Savoie Mont Blanc, INRAE, CARRTEL, Thonon les Bains, France| Laboratory for Aquaculture and Pathology of Aquatic Organisms Division for Marine and Environmental Research Rudjer Boskovic Institute, Zagreb, Croatia¶ MJD Consulting, Croatia, Hvar, Croatia# University of Zagreb, Zagreb, Croatia
Corresponding author: Tena Radočaj ( tradocaj@agr.hr ) © Tena Radočaj, Oliver Barić, Serena Rasconi, Irena Vardić, Jurica Jug-Dujaković, Ana Gavrilović. This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation:
Radočaj T, Barić O, Rasconi S, Vardić I, Jug-Dujaković J, Gavrilović A (2024) Steps of DNA extraction for molecular analysis of microalgae communities - a simple guide for beginners. ARPHA Preprints. https://doi.org/10.3897/arphapreprints.e128742 |  |
Abstract
One of the biggest challenges in the microalgae industry is the lack of reliable methods to detect and avoid biological contaminants, including parasites, which can affect cell growth and consequently lead to crash of the culture. In recent years, the metabarcoding approach has been widely used to study diversity and distribution of microalgae communities and is also a promising tool for contaminant detection in routine biomonitoring. The aim of this article presents steps of DNA extraction for molecular analysis of microalgae communities.
Keywords
microalgae communities, DNA extraction, parasites