ARPHA Preprints, doi: 10.3897/arphapreprints.e156971
Diverse metabolites with anti-psoriasis potential from different fermentations of the fungicolous fungus Xylaria longipes HFG1018
Zhen-Zhu Zhao‡, Yan Wang‡, Xiao-Yu Wang‡, Hui Chen‡, Zhen-Zhen Wang‡, Jing-Kun Wang‡, Le-Le Wang‡, Ming-Jun Shen‡, Xin Pang‡, Wei-Sheng Feng‡‡ Henan University of Chinese Medicine, Zhengzhou, China
Corresponding author: Zhen-Zhu Zhao ( zzhenzhu0921@163.com ) Corresponding author: Xin Pang ( pangxin116@163.com ) Corresponding author: Wei-Sheng Feng ( fwsh@hactcm.edu.cn ) © Zhen-Zhu Zhao, Yan Wang, Xiao-Yu Wang, Hui Chen, Zhen-Zhen Wang, Jing-Kun Wang, Le-Le Wang, Ming-Jun Shen, Xin Pang, Wei-Sheng Feng. This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation:
Zhao Z-Z, Wang Y, Wang X-Y, Chen H, Wang Z-Z, Wang J-K, Wang L-L, Shen M-J, Pang X, Feng W-S (2025) Diverse metabolites with anti-psoriasis potential from different fermentations of the fungicolous fungus Xylaria longipes HFG1018. ARPHA Preprints. https://doi.org/10.3897/arphapreprints.e156971 |  |
Abstract
The genus Xylaria is a promising source of bioactive compounds. This study explored the diversity of secondary metabolites (SMs) in Xylaria longipes under three fermentation conditions, isolating 14 new SMs and 27 known ones. The structures were elucidated using NMR, HRESIMS, ROESY, and J-based configuration analysis, with absolute configurations determined through calculated ECD and 13C NMR data. A comprehensive library of SMs was constructed, facilitating metabolomics-wide association studies that identified culture conditions as a critical factor influencing SM production. This compound library also simplifies the determination of absolute configurations for diplosporins by analyzing J values and CD trends. Anti-proliferative assays against induced T/B lymphocytes and HaCaT cells revealed that more than half of the compounds exhibited significant inhibitory activity, with compounds 2, 15, and 32 reducing IFN-γ secretion. Compound 32 showed promising anti-psoriatic effects by inhibiting NF-κB p65 phosphorylation in HaCaT cells. This first systematic chemical study of X. longipes under varied conditions provides insights into structure-activity relationships.
Keywords
Xylaria longipes, Xylariaceae, Different fermentation conditions, Chemical investigation, Immunosuppression, HaCaT cells, Psoriasis