ARPHA Preprints, doi: 10.3897/arphapreprints.e65983
DNA barcodes from northern birds of Colombia
expand article infoPaulo Cesar Pulgarín-R, Martha Olivera-Angel§, Luisa Ortíz§, Duván Nanclares§, Sara Velásquez-Restrepo|, Juan Fernando Diaz-Nieto
‡ Facultad de Ciencias y Biotecnología, Universidad CES, Medellín, Colombia§ Biogénesis, Facultad de Ciencias Agrarias, Universidad de Antioquia, Cl. 73 #73A-79, Medellín, Colombia| Grupo Biodiversidad, Evolución y Conservación (BEC), Departamento de Ciencias Biológicas, Escuela de Ciencias, Universidad EAFIT, Carrera 49 No. 7 sur-50, Medellín, Colombia¶ Universidad EAFIT, Medellin, Colombia
Open Access

DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation bird species from northern Colombia with the goal to provide tools for species identification in this region of South America. We blood sampled birds in a lowland tropical forest with various degrees of intervention using standard 10 × 12 mist-nets. We extracted DNA and sequenced the COI barcode gene using standard primers and laboratory methods. We obtained 28 COI sequences from 19 species, 10 families and 3 orders and found that barcodes largely matched (but not always) phenotypic identification (>90%) and they also facilitated the identification of several challenging passerine species. Despite our reduced sampling, our study represents the first attempt to document COI barcodes for birds (from blood samples) in this part of Colombia, which fills a considerable gap of sampling in this part of South America.

Aves, lowland tropical forest, mtDNA, northern Colombia,