ARPHA Preprints, doi: 10.3897/arphapreprints.e70782
Gene Sequence Analysis of Mitochondrial COI in Genus Saperda (Coleoptera: Cerambycidae: Lamiinae)
expand article infoWei XU§, Qinhua Gan|, Jian Pu, Yingwen Pan|, Bo Cai|, Su Ao§, Rui Meng§, Li Chen#
‡ College of Plant Protection,Southwest University, Chongqing, China§ Post-Entry Quarantine Station for Tropical Plants of Haikou Customs, Haikou, China| Doctor, Haikou City, China¶ HNPOLY PHARM. CO.,LTD, Haikou, China# College of Plant Protection, Southwest University, Chongqing, China
Open Access
Abstract

In this study, the total DNA of nine species of Saperda (Lopezcolonia) octopunctata (Scopoli, 1772), Saperda (Lopezcolonia) scalaris (Linnaeus, 1758), Saperda interrupta Gebler, Saperda Alberti (Plavilstshikov), Saperda (Saperda) similis Laicharting, 1784, Saperda (Compsidia) populnea (Linnaeus, 1758), Saperda (Saperda) carcharias (Linnaeus, 1758), Saperda (Lopezcolonia) perforata Pallas, 1773 and Saperda ohbayashi were extracted. Two partial sequences of mitochondrial gene and one partial sequence of nuclear gene were amplified. Comparing the COI sequence with the DNA barcode data in GenBank can effectively identify the related species of Saperda. It will be applied to the rapid identification of some species of Saperda in imported wood at ports, and improve the detection rate of plant quarantine.

Keywords
Genus Saperda; mtDNA; COI; sequence analysis