Corresponding author: Wei XU ( 840252833@qq.com ) Corresponding author: Rui Meng ( 541946422@qq.com ) Corresponding author: Li Chen ( lichen57@swu.edu.cn ) © Wei XU, Qinhua Gan, Jian Pu, Yingwen Pan, Bo Cai, Su Ao, Rui Meng, Li Chen. This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation:
XU W, Gan Q, Pu J, Pan Y, Cai B, Ao S, Meng R, Chen L (2021) Gene Sequence Analysis of Mitochondrial COI in Genus Saperda (Coleoptera:Cerambycidae:Lamiinae). ARPHA Preprints. https://doi.org/10.3897/arphapreprints.e70782 |
In this study, the total DNA of nine species of Saperda (Lopezcolonia) octopunctata (Scopoli, 1772), Saperda (Lopezcolonia) scalaris (Linnaeus, 1758), Saperda interrupta Gebler, Saperda Alberti (Plavilstshikov), Saperda (Saperda) similis Laicharting, 1784, Saperda (Compsidia) populnea (Linnaeus, 1758), Saperda (Saperda) carcharias (Linnaeus, 1758), Saperda (Lopezcolonia) perforata Pallas, 1773 and Saperda ohbayashi were extracted. Two partial sequences of mitochondrial gene and one partial sequence of nuclear gene were amplified. Comparing the COI sequence with the DNA barcode data in GenBank can effectively identify the related species of Saperda. It will be applied to the rapid identification of some species of Saperda in imported wood at ports, and improve the detection rate of plant quarantine.